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The sample is pushed in the sample loop with the assistance from the syringe mechanism. Last of all, the injection valve is rotated to achieve the inject posture so that the cell stage circulation from your pump for the column is directed in the sample loop, and also the sample is injected in to the column.On this technique, the relocating solvent

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. HPLC separation of a mixture of flavonoids with UV/Vis detection at 360 nm and, from the inset, at 260 nm. The selection of wavelength has an effect on Each individual analyte’s sign.A syringe pump may be used for even greater Charge of circulation price; even so, the syringe pump is unable to make as much tension as being a piston pump, so it

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